HEPARIN AND HYPOTAURINE SUPPLEMENTATION IMPROVE THE FERTILIZATION RATE OF SHEEP OOCYTES MATURED IN MEDIA CONTAINING L-CARNITINE IN VITRO

This study to do examine the effectiveness of using heparin and hypotaurine improve in vitro fertilization rate. In first stage, oocytes were matured maturation medium supplemented with 0.3 mg/mL L-carnitine nuclear maturation. second fertilized semen incubated 0.1 IU/mL 10 µM hypotaurine. The final concentration sperm used was 5x106 spermatozoa/mL, its interaction carried out for 12 hours 5% CO2 incubator at 38.5° C. results showed that percentage reached metaphase II significantly higher (P0.05) media compared control (88.2±4.9% vs 79.8±5.1%). Furthermore, rate characterized by formation 2 PN than (82.6±2.0% 73.3±2.4%). conclusion, supplementation dose on effective increasing oocytes. combination also successful elevating